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fluorescein isothiocyanate (fitc)-conjugated rat anti-mouse stem cell antigen (sca1)  (Thermo Fisher)


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    Thermo Fisher fluorescein isothiocyanate (fitc)-conjugated rat anti-mouse stem cell antigen (sca1)
    Fluorescein Isothiocyanate (Fitc) Conjugated Rat Anti Mouse Stem Cell Antigen (Sca1), supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fluorescein isothiocyanate (fitc)-conjugated rat anti-mouse stem cell antigen (sca1)/product/Thermo Fisher
    Average 90 stars, based on 1 article reviews
    fluorescein isothiocyanate (fitc)-conjugated rat anti-mouse stem cell antigen (sca1) - by Bioz Stars, 2026-03
    90/100 stars

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    (A) Thyroid cancer mouse model: TPO-Cre/LSL-BrafV600E/p53 lox-lox /eYFP. (B) Representative images of thyroid tumors in the ATC mouse model at 6-8 and 18-21 weeks of age (Scale bar, 1cm). (C) Images of H&E staining of thyroid tumor section from ATC mouse model at 6-8 and 18-21 weeks (Scale bar, 100 μ m). (D) Tumor thyroid weights from ATC mice at 6-8 and 18-21 weeks. Each dot represents an individual animal (6-8 weeks, n=6; 18-21 weeks, n=11). (E) D2 and D3 mRNA expression levels in thyroids from ATC mice at different ages compared to normal thyroid tissue. Each point represents the mean of pooled samples from several animals (2-3 weeks, n= 3; 6-8 weeks, n=6; 13-15 weeks, n=7; and 18-21 weeks, n= 11). (F) Representative blow cytometry dot plot showing YFP + (green) and YFP − (red) cells. (G) Percentage of epithelial (YFP + ) and mesenchymal (YFP − ) cells from pooled thyroid tumors of ATC mice at 20 weeks, analyzed by FACS. Each point represents an individual experiment (pooled tumors from n=3 mice). (H) D2 mRNA expression in YFP + and YFP − cells sorted from ATC thyroid tumors at 20 weeks. Each point represents a technical replicate from pooled tumors (n=3 mice). (I) In silico analysis of scRNA-seq data extracted from Lu et al. . (J) Representative blow cytometry dot plot showing YFP + (green) and <t>SCA1</t> + (violet) cells. (K) Percentage of epithelial (YFP + ) and CAF (SCA + ) cells in pooled thyroid tumors from 20-week-old ATC mice, analyzed by FACS. Each point represents an individual experiment (pooled tumors from n=3 mice). (L) D2 mRNA expression in thyroid tumors at 8 and 20 weeks and in YFP + and SCA + and YFP − /SCA1 − cells sorted at 20 weeks. (M-N) Col1a1 (M) and IL6 (N) mRNA expression in thyroid tumors at 8 and 20 weeks and in YFP + and SCA + cells sorted from tumors at 20 weeks. Each point represents an individual experiment. Data are expressed as mean ± S.E.M. * P < 0.05, ** P < 0.01, ***P < 0.0001 using t tests in D, G, H and K or ANOVA in E, L, M and N.
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    (A) Thyroid cancer mouse model: TPO-Cre/LSL-BrafV600E/p53 lox-lox /eYFP. (B) Representative images of thyroid tumors in the ATC mouse model at 6-8 and 18-21 weeks of age (Scale bar, 1cm). (C) Images of H&E staining of thyroid tumor section from ATC mouse model at 6-8 and 18-21 weeks (Scale bar, 100 μ m). (D) Tumor thyroid weights from ATC mice at 6-8 and 18-21 weeks. Each dot represents an individual animal (6-8 weeks, n=6; 18-21 weeks, n=11). (E) D2 and D3 mRNA expression levels in thyroids from ATC mice at different ages compared to normal thyroid tissue. Each point represents the mean of pooled samples from several animals (2-3 weeks, n= 3; 6-8 weeks, n=6; 13-15 weeks, n=7; and 18-21 weeks, n= 11). (F) Representative blow cytometry dot plot showing YFP + (green) and YFP − (red) cells. (G) Percentage of epithelial (YFP + ) and mesenchymal (YFP − ) cells from pooled thyroid tumors of ATC mice at 20 weeks, analyzed by FACS. Each point represents an individual experiment (pooled tumors from n=3 mice). (H) D2 mRNA expression in YFP + and YFP − cells sorted from ATC thyroid tumors at 20 weeks. Each point represents a technical replicate from pooled tumors (n=3 mice). (I) In silico analysis of scRNA-seq data extracted from Lu et al. . (J) Representative blow cytometry dot plot showing YFP + (green) and <t>SCA1</t> + (violet) cells. (K) Percentage of epithelial (YFP + ) and CAF (SCA + ) cells in pooled thyroid tumors from 20-week-old ATC mice, analyzed by FACS. Each point represents an individual experiment (pooled tumors from n=3 mice). (L) D2 mRNA expression in thyroid tumors at 8 and 20 weeks and in YFP + and SCA + and YFP − /SCA1 − cells sorted at 20 weeks. (M-N) Col1a1 (M) and IL6 (N) mRNA expression in thyroid tumors at 8 and 20 weeks and in YFP + and SCA + cells sorted from tumors at 20 weeks. Each point represents an individual experiment. Data are expressed as mean ± S.E.M. * P < 0.05, ** P < 0.01, ***P < 0.0001 using t tests in D, G, H and K or ANOVA in E, L, M and N.
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    (A) Thyroid cancer mouse model: TPO-Cre/LSL-BrafV600E/p53 lox-lox /eYFP. (B) Representative images of thyroid tumors in the ATC mouse model at 6-8 and 18-21 weeks of age (Scale bar, 1cm). (C) Images of H&E staining of thyroid tumor section from ATC mouse model at 6-8 and 18-21 weeks (Scale bar, 100 μ m). (D) Tumor thyroid weights from ATC mice at 6-8 and 18-21 weeks. Each dot represents an individual animal (6-8 weeks, n=6; 18-21 weeks, n=11). (E) D2 and D3 mRNA expression levels in thyroids from ATC mice at different ages compared to normal thyroid tissue. Each point represents the mean of pooled samples from several animals (2-3 weeks, n= 3; 6-8 weeks, n=6; 13-15 weeks, n=7; and 18-21 weeks, n= 11). (F) Representative blow cytometry dot plot showing YFP + (green) and YFP − (red) cells. (G) Percentage of epithelial (YFP + ) and mesenchymal (YFP − ) cells from pooled thyroid tumors of ATC mice at 20 weeks, analyzed by FACS. Each point represents an individual experiment (pooled tumors from n=3 mice). (H) D2 mRNA expression in YFP + and YFP − cells sorted from ATC thyroid tumors at 20 weeks. Each point represents a technical replicate from pooled tumors (n=3 mice). (I) In silico analysis of scRNA-seq data extracted from Lu et al. . (J) Representative blow cytometry dot plot showing YFP + (green) and <t>SCA1</t> + (violet) cells. (K) Percentage of epithelial (YFP + ) and CAF (SCA + ) cells in pooled thyroid tumors from 20-week-old ATC mice, analyzed by FACS. Each point represents an individual experiment (pooled tumors from n=3 mice). (L) D2 mRNA expression in thyroid tumors at 8 and 20 weeks and in YFP + and SCA + and YFP − /SCA1 − cells sorted at 20 weeks. (M-N) Col1a1 (M) and IL6 (N) mRNA expression in thyroid tumors at 8 and 20 weeks and in YFP + and SCA + cells sorted from tumors at 20 weeks. Each point represents an individual experiment. Data are expressed as mean ± S.E.M. * P < 0.05, ** P < 0.01, ***P < 0.0001 using t tests in D, G, H and K or ANOVA in E, L, M and N.
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    (A) Thyroid cancer mouse model: TPO-Cre/LSL-BrafV600E/p53 lox-lox /eYFP. (B) Representative images of thyroid tumors in the ATC mouse model at 6-8 and 18-21 weeks of age (Scale bar, 1cm). (C) Images of H&E staining of thyroid tumor section from ATC mouse model at 6-8 and 18-21 weeks (Scale bar, 100 μ m). (D) Tumor thyroid weights from ATC mice at 6-8 and 18-21 weeks. Each dot represents an individual animal (6-8 weeks, n=6; 18-21 weeks, n=11). (E) D2 and D3 mRNA expression levels in thyroids from ATC mice at different ages compared to normal thyroid tissue. Each point represents the mean of pooled samples from several animals (2-3 weeks, n= 3; 6-8 weeks, n=6; 13-15 weeks, n=7; and 18-21 weeks, n= 11). (F) Representative blow cytometry dot plot showing YFP + (green) and YFP − (red) cells. (G) Percentage of epithelial (YFP + ) and mesenchymal (YFP − ) cells from pooled thyroid tumors of ATC mice at 20 weeks, analyzed by FACS. Each point represents an individual experiment (pooled tumors from n=3 mice). (H) D2 mRNA expression in YFP + and YFP − cells sorted from ATC thyroid tumors at 20 weeks. Each point represents a technical replicate from pooled tumors (n=3 mice). (I) In silico analysis of scRNA-seq data extracted from Lu et al. . (J) Representative blow cytometry dot plot showing YFP + (green) and <t>SCA1</t> + (violet) cells. (K) Percentage of epithelial (YFP + ) and CAF (SCA + ) cells in pooled thyroid tumors from 20-week-old ATC mice, analyzed by FACS. Each point represents an individual experiment (pooled tumors from n=3 mice). (L) D2 mRNA expression in thyroid tumors at 8 and 20 weeks and in YFP + and SCA + and YFP − /SCA1 − cells sorted at 20 weeks. (M-N) Col1a1 (M) and IL6 (N) mRNA expression in thyroid tumors at 8 and 20 weeks and in YFP + and SCA + cells sorted from tumors at 20 weeks. Each point represents an individual experiment. Data are expressed as mean ± S.E.M. * P < 0.05, ** P < 0.01, ***P < 0.0001 using t tests in D, G, H and K or ANOVA in E, L, M and N.
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    Image Search Results


    (A) Thyroid cancer mouse model: TPO-Cre/LSL-BrafV600E/p53 lox-lox /eYFP. (B) Representative images of thyroid tumors in the ATC mouse model at 6-8 and 18-21 weeks of age (Scale bar, 1cm). (C) Images of H&E staining of thyroid tumor section from ATC mouse model at 6-8 and 18-21 weeks (Scale bar, 100 μ m). (D) Tumor thyroid weights from ATC mice at 6-8 and 18-21 weeks. Each dot represents an individual animal (6-8 weeks, n=6; 18-21 weeks, n=11). (E) D2 and D3 mRNA expression levels in thyroids from ATC mice at different ages compared to normal thyroid tissue. Each point represents the mean of pooled samples from several animals (2-3 weeks, n= 3; 6-8 weeks, n=6; 13-15 weeks, n=7; and 18-21 weeks, n= 11). (F) Representative blow cytometry dot plot showing YFP + (green) and YFP − (red) cells. (G) Percentage of epithelial (YFP + ) and mesenchymal (YFP − ) cells from pooled thyroid tumors of ATC mice at 20 weeks, analyzed by FACS. Each point represents an individual experiment (pooled tumors from n=3 mice). (H) D2 mRNA expression in YFP + and YFP − cells sorted from ATC thyroid tumors at 20 weeks. Each point represents a technical replicate from pooled tumors (n=3 mice). (I) In silico analysis of scRNA-seq data extracted from Lu et al. . (J) Representative blow cytometry dot plot showing YFP + (green) and SCA1 + (violet) cells. (K) Percentage of epithelial (YFP + ) and CAF (SCA + ) cells in pooled thyroid tumors from 20-week-old ATC mice, analyzed by FACS. Each point represents an individual experiment (pooled tumors from n=3 mice). (L) D2 mRNA expression in thyroid tumors at 8 and 20 weeks and in YFP + and SCA + and YFP − /SCA1 − cells sorted at 20 weeks. (M-N) Col1a1 (M) and IL6 (N) mRNA expression in thyroid tumors at 8 and 20 weeks and in YFP + and SCA + cells sorted from tumors at 20 weeks. Each point represents an individual experiment. Data are expressed as mean ± S.E.M. * P < 0.05, ** P < 0.01, ***P < 0.0001 using t tests in D, G, H and K or ANOVA in E, L, M and N.

    Journal: bioRxiv

    Article Title: Type 2 Deiodinase in Cancer-Associated Fibroblasts is required to sustain growth of poorly and undifferentiated thyroid cancer

    doi: 10.1101/2025.07.10.664131

    Figure Lengend Snippet: (A) Thyroid cancer mouse model: TPO-Cre/LSL-BrafV600E/p53 lox-lox /eYFP. (B) Representative images of thyroid tumors in the ATC mouse model at 6-8 and 18-21 weeks of age (Scale bar, 1cm). (C) Images of H&E staining of thyroid tumor section from ATC mouse model at 6-8 and 18-21 weeks (Scale bar, 100 μ m). (D) Tumor thyroid weights from ATC mice at 6-8 and 18-21 weeks. Each dot represents an individual animal (6-8 weeks, n=6; 18-21 weeks, n=11). (E) D2 and D3 mRNA expression levels in thyroids from ATC mice at different ages compared to normal thyroid tissue. Each point represents the mean of pooled samples from several animals (2-3 weeks, n= 3; 6-8 weeks, n=6; 13-15 weeks, n=7; and 18-21 weeks, n= 11). (F) Representative blow cytometry dot plot showing YFP + (green) and YFP − (red) cells. (G) Percentage of epithelial (YFP + ) and mesenchymal (YFP − ) cells from pooled thyroid tumors of ATC mice at 20 weeks, analyzed by FACS. Each point represents an individual experiment (pooled tumors from n=3 mice). (H) D2 mRNA expression in YFP + and YFP − cells sorted from ATC thyroid tumors at 20 weeks. Each point represents a technical replicate from pooled tumors (n=3 mice). (I) In silico analysis of scRNA-seq data extracted from Lu et al. . (J) Representative blow cytometry dot plot showing YFP + (green) and SCA1 + (violet) cells. (K) Percentage of epithelial (YFP + ) and CAF (SCA + ) cells in pooled thyroid tumors from 20-week-old ATC mice, analyzed by FACS. Each point represents an individual experiment (pooled tumors from n=3 mice). (L) D2 mRNA expression in thyroid tumors at 8 and 20 weeks and in YFP + and SCA + and YFP − /SCA1 − cells sorted at 20 weeks. (M-N) Col1a1 (M) and IL6 (N) mRNA expression in thyroid tumors at 8 and 20 weeks and in YFP + and SCA + cells sorted from tumors at 20 weeks. Each point represents an individual experiment. Data are expressed as mean ± S.E.M. * P < 0.05, ** P < 0.01, ***P < 0.0001 using t tests in D, G, H and K or ANOVA in E, L, M and N.

    Article Snippet: The blow through fraction was collected and incubated with an anti-Sca1-microbeads antibody (Miltenyi Biotec #130–106– 641).

    Techniques: Staining, Expressing, Cytometry, In Silico